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HLA T & B CELL FLOW CYTOMETRY CROSSMATCH Test in Lucknow

Test Recommended For:
N/A

Report Frequency :
Sample Daily by 9 am; Report Next day

Parameters :
10


Parameters :
10
- ₹ 4040
- 50+

Quality Checks & Processes On Every Sample
15+Quality Indicator Monitored On Daily Basis
Overview
Cross match is the process of matching the donor with the recipient (patient) in patients requiring organ transplant such as kidney, liver, lung, heart & bone marrow. Human leukocyte antigens (HLA) present on the surface of lymphocytes (white blood cells) in the donor's blood is tested against the blood taken from the patient. Cross match is positive If any reaction is seen. A negative cross match means that you can go ahead with the transplant. This test is performed by a highly sensitive flow cytometry technique.
Parameters
Doctor Information

Pre-test Information
Collect specimen preferably 48 hours after dialysis. Doctor's prescription on the letter head with stamp AND 1 photograph each of recipient and donor is mandatory. Brief history in Solid Organ Transplant Consent form (Form 33) is mandatory.

Report Delivery
Sample Daily by 9 am; Report Next day

Code
L033

Category
Transplantation

Stability Room
24 hrs

Stability Refrigerated
NA

Stability Frozen
NA

Method
Flow Cytometry

Usage
For patients requiring solid organ transplants like liver or kidney, Flow cytometry crossmatch (FCXM) is set up between recipient and donor to check for the presence of Anti HLA IgG antibodies. This may be done both pre and post transplant.

Specimen
RECIPIENT: 2 mL (1 mL min.) Serum from 1 SST AND 4 mL (2 mL min.) Whole blood from 1 Green Top (Sodium Heparin) tube. DONOR: 3 mL (2 mL min.) Whole blood in 1 Green Top (Sodium Heparin) tube. Ship immediately at 18- 22 ͦ C. DO NOT FREEZE. Brief history in Solid Organ Transplant Consent form (Form 33) is mandatory. Doctor's prescription on the letter head with stamp AND 1 photograph each of recipient and donor is mandatory. Above specimen requirements are for a single donor being cross matched with a single receipient. For additional cross matches, send additional specimens of donors. Request as additional tests. Collect specimen preferably 48 hours after dialysis.
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