HLA T & B CELL FLOW CYTOMETRY CROSSMATCH Test in Agra

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    Test Recommended For:

    N/A

  • Report Frequency :

    Sample Daily by 9 am; Report Next day

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  • parameter icon

    Parameters :

    10

Special Instruction :
Collect specimen preferably 48 hours after dialysis. Doctor's prescription on the letter head with stamp AND 1 photograph each of recipient and donor is mandatory. Brief history in Solid Organ Transplant Consent form (Form 33) is mandatory.

Overview

Cross match is the process of matching the donor with the recipient (patient) in patients requiring organ transplant such as kidney, liver, lung, heart & bone marrow. Human leukocyte antigens (HLA) present on the surface of lymphocytes (white blood cells) in the donor's blood is tested against the blood taken from the patient. Cross match is positive If any reaction is seen. A negative cross match means that you can go ahead with the transplant. This test is performed by a highly sensitive flow cytometry technique.

Parameters

Doctor Information

Code

Pre-test Information

Collect specimen preferably 48 hours after dialysis. Doctor's prescription on the letter head with stamp AND 1 photograph each of recipient and donor is mandatory. Brief history in Solid Organ Transplant Consent form (Form 33) is mandatory.

Code

Report Delivery

Sample Daily by 9 am; Report Next day

Code

Code

L033

Category

Category

Transplantation

Stability Room

Stability Room

24 hrs

Stability Refrigerated

Stability Refrigerated

NA

Stability Frozen

Stability Frozen

NA

Method

Method

Flow Cytometry

Specimen

Usage

For patients requiring solid organ transplants like liver or kidney, Flow cytometry crossmatch (FCXM) is set up between recipient and donor to check for the presence of Anti HLA IgG antibodies. This may be done both pre and post transplant.

Specimen

Specimen

RECIPIENT: 2 mL (1 mL min.) Serum from 1 SST AND 4 mL (2 mL min.) Whole blood from 1 Green Top (Sodium Heparin) tube. DONOR: 3 mL (2 mL min.) Whole blood in 1 Green Top (Sodium Heparin) tube. Ship immediately at 18- 22 ͦ C. DO NOT FREEZE. Brief history in Solid Organ Transplant Consent form (Form 33) is mandatory. Doctor's prescription on the letter head with stamp AND 1 photograph each of recipient and donor is mandatory. Above specimen requirements are for a single donor being cross matched with a single receipient. For additional cross matches, send additional specimens of donors. Request as additional tests. Collect specimen preferably 48 hours after dialysis.

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