IMMUNOPHENOTYPING BY FLOW CYTOMETRY: CD13 Test in Bengaluru

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    Test Recommended For:

    Male, Female

  • Report Frequency :

    Sample Daily by 9 am; Report Same day

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  • parameter icon

    Parameters :

    27

Special Instruction :
Give brief clinical history.

Overview

Flow cytometry is a technique that rapidly performs analysis of single cells that are suspended in a solution by using lasers. It identifies markers present on the surface of the cells as well as inside the cells. Commonly this technique is used to type the cancers arising from blood & bone marrow called Leukemias and Lymphomas. By this method leukemias / lymphomas can be typed as per their origin from different types of white blood cells namely B-lymphocytes, T-lymphocytes, NK cells & other cells like histiocytes. CD13 positivity is seen in Acute Myeloid Leukemia (AML) arising from myeloid cells, a type of white blood cell.

Parameters

Doctor Information

Code

Pre-test Information

Give brief clinical history.

Code

Report Delivery

Sample Daily by 9 am; Report Same day

Code

Code

H143

Category

Category

Cancer

Stability Room

Stability Room

24 hrs

Stability Refrigerated

Stability Refrigerated

NA

Stability Frozen

Stability Frozen

NA

Method

Method

Flow Cytometry

Specimen

Usage

CD13 is a myeloid cell marker. Flow cytometric pattern of CD13, CD15 & CD34 positivity is seen in Acute myeloid leukemia (AML). CD13 differentiates AML M0 (CD13 positive) from ALL (CD13 negative).

Specimen

Specimen

3 mL (2 mL min.) Whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) Whole blood in 1 Green Top (Sodium Heparin) tube OR 2 mL (1 mL min.) Bone marrow in 1 Green Top (Sodium heparin) tube. Ship immediately at 18–22°C. DO NOT REFRIGERATE OR FREEZE. Specify time, date and clinical details on test request form.

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