1.The assay is used for the quantitative detection of IgG antibodies against the toxin of Clostridium tetani in serum samples. 2.Positive results indicates presence of Tetanus toxoid-IgG-antibody in patient’s sample. It helps in determining the necessity of vaccination and for checking the success of vaccination. 3.Negative results indicates absence of Tetanus toxoid -IgG-antibody in patient’s sample. It may be as a result of lack of or no response to vaccination.