Isoelectric focusing is the gold standard technique with greatest sensitivity & specificity for diagnosis of Multiple sclerosis. Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease characterized by visual, motor & Multiple sclerosis (MS) is an autoimmune disease of CNS characterized by chronic inflammation, demyelination, gliosis & neuronal loss. The disease course can be relapsing or progressive. It is 3 fold more common in women than men and usually presents between 20 to 40 years of age. Well established risk factors are genetic predisposition, vitamin D deficiency, Epstein-Barr virus infection after early childhood & smoking.