1. This assay is used for qualitative detection of specific IgM antibodies to Herpes Simplex virus 2 in serum samples. 2. Positive result for Herpes Simplex virus 2 IgM may indicate acute infection, reinfection or reactivation of latent virus. Persistence of low level HSV 2 IgM antibodies following post infection over a long period is not uncommon. 3. Negative result for Herpes Simplex virus 2 IgM indicates no Herpes Simplex virus 2 infection. False negative reaction may be due to processing of sample collected early in the course of disease or absence of immune response.