||2 mL (1 mL min.) plasma from 1 Grey Top (Sodium Fluoride) tube AND 10 mL (5 mL min.) aliquot of corresponding urine for each timed specimen. Collect fasting baseline plasma and urine specimen (0 hr). Dissolve 82.5 g glucose monohydrate (equivalent to 75 g anhydrous glucose) in adults or 1.92 g / Kg body wt in children in 300 mL water. Administer orally over a period of 5 minutes. Collect additional plasma and urine samples at 0.5, 1.0, 1.5, 2.0 & 2.5 hours. Note time drawn on each tube, urine vial and test request form. Specify amount of glucose administered on test request form. Overnight fasting mandatory. Ship refrigerated or frozen.