||3 mL (1.5 mL min.) Platelet Poor Plasma (PPP) from 2 Blue Top (Sodium Citrate) tubes. Prepare PPP within 1 hour of collection as follows: Centrifuge sample at 3600 rpm for 15 min & transfer supernatent to a clean plastic tube. Centrifuge this supernatant again at 3600 rpm for 15 mins & finally transfer the supernatant (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory Patient should avoid Anabolic steroids, Gemfibrozil, Heparin therapy, Asparaginase, Estrogens, Gestodene and Oral contraceptives 3 days prior to specimen collection.