Dr. Priyanka Agarwal

Research Scientist

Molecular Diagnostics, R& D

National Reference Laboratory

Dr Lal PathLabs Ltd

Qualification:

  • Ph.D., South Campus, Delhi University, New Delhi, India
  • M.Phil, South Campus, Delhi University, New Delhi, India
  • P.G. Diploma in Bio-Informatics, Banasthali Vidyapith (Rajasthan)

Expertise:

  • Genomic DNA Isolation (Dellaporta method and c-TAB method),
  • RNA Isolation (Trizol, Hot Phenol and GITC Method),
  • Protein isolation, expression and purification,
  • Molecular Cloning (Antisense, Overexpression, RNAi, Gateway Cloning),
  • Promoter characterization, Extraction and purification of plasmids (Mini and Midi preparations), Ligation, Transformation,
  • Restriction Digestion and high throughput sequencing analysis,
  • DNA and RNA estimation (by Nanodrop, Bioanalyzer, Gel detection), PCR, Colony PCR, RT-PCR, Semi quantitative PCR,
  • Southern and Northern hybridization (DIG Labeling methods and Radioactivity), Microarray.

Key Responsibilities:

  • Bioinformatics Analysis.

Trainings:

  • ISRFG International Conference conducted by by DBT in 2013
  • Confocal microscopy workshop
  • Intellectual Property Rights Workshop
  • R analytics coursework conducted by Delhi University Botanical Society.
  • National Symposium on Database Development and Biocuration.

Projects Handled:

  • Characterization of regulatory networks of transcription factors showing cultivar specific drought response in rice
  • Analysis of genomic, epigenome of indica rice varieties N22 and IR64
  • Manipulation of fruit ripening by phase specific gene silencing- a case study with tomato LeEIL1 and LeEIL3
  • Over-expression of cold-induced genes encoding for mitogen activated protein kinase 3 and alternative oxidase 1 in tomato to improve cold-tolerance

Awards:

  • Received the National Post-Doctoral Fellowship by the Science and Engineering Research Board (SERB-2017)
  • UGC research Junior Research Fellowship for meritorious students (JRF)
  • UGC research Senior Research Fellowship for meritorious students (SRF)
  • Best student of the school (1994)
  • DBT sponsored student in M.Sc program
  • Attended several national and international seminars in India

Research & Publications:

  • Balyan, Sonia; Kumar, Mukesh; Mutum, Roseeta; Raghuvanshi, Utkarsh; Agarwal, Priyanka; Mathur, Saloni; Raghuvanshi, Saurabh. Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22. (Accepted, Scientific Reports, 2017, doi:10.1038/s41598-017-15450-1)
  • Priyanka Agarwal, Rahul Kumar, Amit Pareek and Arun K Sharma (2016).. Fruit preferential activity of a tomato ,RIP1 gene promoter in transgenic tomato and Arabidopsis Mol Genet Genomics (2016). doi:10.1007/s00438-016-1262-4
  • Pankaj Gupta, Preeti Sharma, Kumari Shanno, Vivek Jain, Ashutosh Pareek, Priyanka Agarwal, Randhir Singh, Veena Sharma (2016). Nephroprotective role of alcoholic extract of Pterocarpus marsupium heartwood against experimentally induced diabetic nephropathy. Journal of Pharmacy & Pharmacognosy Research 4 (5): 174-186
  • Pankaj Gupta, Vivek jain, Ashutosh Pareek, Randhir Singh, Priyanka Agarwal, Veena Sharma (2016). Evaluation of effect of alcoholic extract of heartwood of Pterocarpus marsupium on in vitro antioxidant, anti-glycation, sorbitol accumulation and inhibition of aldose reductase activity. Journal of traditional and complementary medicine 17:1-8.
  • Kumar R, Agarwal P, Pareek A, Tyagi AK, Sharma AK (2015) Genomic survey, gene expression, and interaction analysis suggest diverse roles of ARF and Aux/IAA proteins in Solanaceae. Plant Mol Biol Rep 33:1552–1572
  • Kumar R, Agarwal P, Tyagi AK, Sharma AK (2012). Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 genes during development and response to different stimuli in tomato (Solanum lycopersicum). Mol. Genet. Genomics 287: 221–235
  • Priyanka Agarwal, Pratibha Gour, Santosh Kumar, Bhuvneshwar Mishra, Deepika Sharma, Saloni Mathur and Saurabh Raghuvanshi. Comparative transcriptional profiling of tolerant and sensitive rice cultivars in response to drought. (Communicated)
  • Pratibha Gour, Priyanka Agarwal and Saurabh Raghuvanshi. Identification and characterization of transcription factors showing variety specific drought response in rice. (Under preparation)

Book Chapter:

  • Agarwal, P., Jiwani, G., Khurana, A., Gupta, P. and Kumar, R. (2017) Ethylene and Stress Mediated Signaling in Plants: A Molecular Perspective, in Mechanism of Plant Hormone Signaling under Stress (ed G. K. Pandey), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118889022.ch12

Posters:

  • Saloni mathur, Priyanka agarwal, Santosh kumar and Saurabh Raghuvanshi. Comparative genomics, epigenomics and transcriptomics of drought tolerant and sensitive indica rice cultivars. Plant Biology 2014 International Conference. Portland, Oregon, USA
  • Priyanka agarwal, Saloni mathur, Santosh kumar and Saurabh Raghuvanshi. Comparative genomics and transcriptomics of drought sensitive and tolerant indica rice cultivars.. International Symposium of rice functional genoomics, ISRFG-2013. International Conference, New Delhi, India
  • Rahul Kumar, Priyanka Agarwal, Amit pareek, Akhilesh K. Tyagi and Arun K Sharma. The ARF and Aux/IAA complement of Solanaceae: Comprehensive expression profiling and interaction analysis suggest coaction among several members of the two classes of proteins controls fruit development and ripening in tomato. Solanacae International conference, SOL-2013. China
  • Rahul Kumar, Priyanka Agarwal, Akhilesh K. Tyagi and Arun K Sharma.Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 genes during development and response to different stimuli in tomato (Solanum lycopersicum). Solanacae conference, SOL-2012. International Conference. Switzerland