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Dr. Isha Chaudhary
National Reference Laboratory
Dr Lal PathLabs Ltd
Ph.D., Department of Reproductive Biology, AIIMS, New Delhi, India
Genomics and Cytogenetics:
Genomic DNA and plasmid DNA isolation, Agarose gel electrophoresis, Nick Translation, PCR, Interphase FISH and Metaphase FISH, Embryo PCR (Nested) & Embryo FISH, Microscopic Analysis of FISH Signals, Primed Insitu Hybridisation (PRINS), Yq microdeletion PCR, Quantitative Fluorescent PCR, Karyotyping. Under the supervision of Dr. Halder, Professor and Head,Dept of Reproductive Biology, AIIMS; I have worked on probe FISH on sperm cells, cancer tissue, prenatal samples and microdeletion syndromes like Di-George, PraderWilli, Retinoblastoma, Miller Decker, William syndrome etc.
Statistical methods and Computer knowledge:
SPSS, Graph pad Prism. Tools: MS Office (Word, Excel & PowerPoint) etc.
Biochemical assays and Protein Techniques:
Panel reactive antibody test, Native and SDS-PAGE, Western blot analysis, Immunohistochemistry (IHC), ELISA.
Works involves routine chromosome diagnosis for infertility, patients with short stature, hypogonadism, undescended testis, microcephaly, delayed developmental milestones, familial and isolated mental retardation, and cerebral palsy. Screening of 10 cases of constitutional abnormalities per day and their reporting as per ISCN nomenclature.
Planning & implementing of internal Quality Control, External Quality Control & proficiency tests.
Validation of cytogenetics reports pertaining to normal or any structural or numerical chromosomal anomalies.
Ensure results are reported within turnaround time.
Complaint handling & reducing complaints.
Additionally, shaping up the data generated form the laboratory into a research articles.
Area of Interest:
Molecular pathology diagnostics
Qualified GATE -2007
Qualified ICMR JRF -2007
Research & Publications:
Communicated: An investigation on biological basis of skewed sex ratio in mouse model
Chaudhary I, Jain M and Halder A. Sperm Sex Ratio (X: Y Ratio) and its Variations. Austin J Reprod Med Infertil 2014; 1(1): 7.
Halder A, Jain M, Chaudhary I. Suspected microdeletion syndromes and molecular cytogenetic techniques: an experience with 330 cases. Molecular Cytogenetics 2014, 7(Suppl 1):O7.
Halder A, Jain M, Chaudhary I, Kumar G, Das T & Gupta YK. Dark-coloured semen in nonobstructive azoospermia: a report of four cases. Andrologia 2013: 1–6, doi.10.1111/ and 12078.
Halder A, Jain M, Chaudhary I, Gupta N and Kabra M. Fluorescence in situ hybridization (FISH) using non-commercial probes in the diagnosis of clinically suspected microdeletion syndromes. Indian J Med Res 138, July 2013: 135-142.
Jain M, Mohan V, Chaudhary I, Halder A. Sertoli Cell Only Syndrome and Glaucoma in a Sex – Determining Region Y (SRY) Positive XX Infertile Male. Journal of Clinical and Diagnostic Research, July 2013 7(7): 1457-1459.
Halder A, Jain M, Chaudhary I. Rapid detection of chromosome X, Y, 13, 18, and 21 aneuploidies by primed in situ labeling/synthesis technique. Indian Journal of Human Genetics. January-March 2013 Volume 19 Issue 1:14-17.
Halder A, Jain M, Chaudhary I and Varma B. Chromosome 22q11.2 microdeletion in monozygotic twins with discordant phenotype and deletion size. Molecular Cytogenetics 2012, 5:13.
Halder A, Jain M, Chaudhary I, Kabra M. Prevalence of 22q11.2 microdeletion in 146 patients with cardiac malformation in a referral hospital of North India. BMC Medical Genetics 2010; 11: 101.
Certificate of Participation in “International Conference on Molecular and Clinical aspects of Gonadal and Nongonadal actions of Gonadotropins” held in Department of Reproductive Biology, AIIMS in Feb, 2008.
Demonstrator cum tutor in “National Workshop on Molecular Cytogenetics: Rapid Prenatal Diagnosis by Fluorescent In-Situ Hybridization (FISH)” held in Department of Reproductive Biology, AIIMS from November 03-08, 2008.
Certificate of Participation in ISHG 2009 International Symposium on ‘Ethics, Culture and Population Genomics’ & 34th Annual Conference of the Indian Society of Human Genetics. March 17-20, 2009, New Delhi.
Demonstrator cum tutor in “National Workshop on Molecular Cytogenetics: Cancer (Solid Tissue) Cytogenetics by Fluorescent In-Situ Hybridization (FISH)” held in Department of Reproductive Biology, AIIMS from August 24-29, 2009.
Certificate of Participation in “Workshop on Technology in Fertility Regulation” held in AIIMS in Feb, 2010.
Certificate of Participation in “RGCON 2010” organised by Rajiv Gandhi Cancer Institute and Research Centre, Delhi in March, 2010.
Demonstrator cum tutor in “National Workshop on Molecular Cytogenetics: Cancer Cytogenetics (Hematological Malignancy) by Fluorescent In-Situ Hybridization (FISH)” held in Department of Reproductive Biology, AIIMS in December, 2010.
Certificate of Participation in International Symposium on “Cross-talk/Conversations on Human Reproduction, Fertility and Sexuality: Evolution and Science, Philosophy, Art, Myths and Literature” held in Department of Reproductive Biology AIIMS in February, 2011.
Certificate of Participation in “RGCON 2011” organised by Rajiv Gandhi Cancer Institute and Research Centre, Delhi in March, 2011.
Certificate of Participation in XVII Annual Congress of the Society of Andrology, India held in Indian Institute of Technology, Delhi from December 10-12, 2011.
Demonstrator cum tutor in “National Workshop on Molecular Cytogenetics: (Male Reproduction)” held in Department of Reproductive Biology AIIMS from November 26- December 1, 2012.
Presented a poster on “Understanding Dynamics of Prezygotic Sex ratio”. Isha Chaudhary, Pradeep Yadav, Ashutosh Halder in ISSRF, February 19-21, 2012, New Delhi.
Presented a poster on “15q11-13 microdeletion study using non - commercial DNA FISH probe in 44 suspected cases of Prader-Willi/Angelman syndrome”. Isha Chaudhary, Manish Jain, Madhulika Kabra, Ashutosh Halder in ISHG-2012, 37th Annual Conference of the Society of Human Genetics. March 3-5, 2012, Chandigarh.