Molecular Diagnostics

  • The Lab is designed as per International standards and is divided into various areas – Pre PCR, Extraction and Post PCR as per guidelines.
  • Department has team of well experienced & dedicated staff of 20 professionals.
  • Department processes around 400,000 samples annually, which is one of the highest workload for any lab across the globe.
  • Department has more than 250 assays in different segments of the test menu to offer.
  • Lab has state of the art instrumentation.

Instrument & Equipments

IonTorrent Proton, OneTouchES _OneTouch2

Ion Torrent platform is recognized as the world’s first PostLight sequencing technology. Instead of using light as an intermediary, Ion Torrent technology creates a new semiconductor chip—with high-density of micro-machined wells to perform biochemical process in a massively parallel way.

  • Simple: Simple sequencing chemistry based on semi-conductor technology, without optical detection. Low sample requirement.
  • Fast: Sequencing within 2-3 hours, rapid turnaround time (~2 days) from sample to DNA sequences.
  • Flexible: Flexible scaled chips on PGM and Proton systems for different throughput needs.
  • High Accuracy: 99.97%.

Being Used for Oncology NGS tests, including BRCA, 50/Any 3 gene CANCER PANELS, MBFD (NIPT) etc

3500 DX Genetic Analyzer

The 3500 Genetic Analyzer with the 8-capillary design featuring a powerful, integrated data collection that provides real-time assessment.

  • This sequencer allows for a majority of applications to utilize a single polymer and capillary array.
  • The 3500 design boost high performance turn times while providing environmentally controlled results.
  • while retaining the unparalleled desired application versatility.

Used For Sanger Sequencing of Small fragments of DNA

COBAS Taqman 96 Analyzer

Roche COBAS TaqMan 96 Automated DNA Amplification and Detection Analyzer.

  • Fully Automated real-time amplification and detection analyzer
  • A high-capacity 96 sample-format that provides automated real-time amplification and detection of DNA or RNA for up to four assays at the same time.
  • Used For Quantitation HCV, HBV, HIV and CMV

QIA SYMPHONY

  • Innovative, easy-to-use modular system with built-in touchscreen.
  • Purification of DNA and RNA from a wide range of samples.
  • Automatic transfer of eluates to the QIAsymphony AS for assay setup.
  • Continuous sample loading, with bar code reading for sample tracking.

Used For fully integrated automation of complete workflows, from sample preparation to assay setup

QIAcube HT - QIAgility

  • QIAcube HT is a compact benchtop instrument that enables automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology.
  • Users can quickly and easily purify DNA, RNA and miRNA from almost any type of sample – including cells, tissues and food material, as well as from bacteria and viruses in animal samples.
  • Automated protocols and dedicated QIAcube HT purification kits and plasticware increase reliability and convenience and save valuable time. The system provides the same high-quality results as other trusted QIAGEN purification technologies.
  • The QIAgility is a compact benchtop instrument that enables rapid, high-precision setup of PCR experiments with ready-to-use software for ease and convenience.

We are using these instruments for TB PC

QuantStudio 6 Flex Real-Time PCR System

The Applied Biosystems QuantStudio 6 Flex Real-Time PCR System is ideal for laboratories with multiple applications. It is an ideal qPCR platform to accommodate changing future needs.

96-well Fast, 384-well plates can be used.

Majorly used for

  • Gene expression, SNP genotyping, Copy number variation
  • Protein thermal shift, High resolution melt and Pathogen detection

ABI 7500 Real-Time PCR

The ABI 7500 Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use.

The 7500 Real-Time PCR Instrument delivers the performance required for high-quality results in a 96-well format.

  • Accommodates tube strips, which can be capped immediately after pipetting each sample
  • Fast mode completes runs in less than forty minutes
  • Optional standard mode facilitates use of standard length real-time PCR assays without changing thermal cycling parameters
  • 5-color variable excitation enables multiplex assays

Used for the Amplification of the Target Gene Sequences.

Rotor Gene Q

Rotor-Gene Q, real-time PCR cycler, combines multiple optimized design features to provide the outstanding performance and reliable results in diagnostics.

  • Outstanding thermal and optical performance due to rotary format
  • An unmatched optical range spanning UV to infrared wavelengths
  • State-of-the art analyses supported by user-friendly software
  • Low maintenance and maximum convenience due to robust design
  • High performance in multiple applications with QIAGEN assays

Used for the Amplification of the Target Gene Sequences.

VERITI – Thermal Cycler

  • The Applied Biosystems Veriti Thermal Cycler delivers the proven reliability you expect from Applied Biosystems PCR instruments. The VeriFlex temperature control technology inside makes it possible to run up to 6 different temperatures in the same protocol step, providing precise control over your PCR optimization.
  • The Veriti Thermal Cycler now has a 3x faster processor, making the easy-to-use color touch screen, more responsive and the time to boot-up, quicker.
  • Used for the Amplification of the Target Gene Sequences.

Thermal Cycler ABI 2720

These 96-well Applied Biosystems thermal cyclers are ideal for both basic PCR and cycle sequencing applications using 0.2 mL reaction tubes or 96-well reaction plates.

  • Proven PCR performance at an affordable price
  • Save lab space without sacrificing results
  • Enhance productivity with intuitive software

Used for Amplification of the desired DNA fragment

TwinCubator

  • The TwinCubator (Hain Lifescience)is intended for use with DNA strips corresponding to reverse hybridization assays and designed for molecular diagnostic assays developed by Hain Lifescience (MTBDRplus).
  • The TwinCubator offers laboratories a rapid and simple hybridization platform for analysing genotyping.
  • Used to perform Line Probe Assays (LPA) for detection of First and Second line Drug Sensitivity in Mycobacterium tuberculosis

Gen-Probe Leader 50i Luminometer

  • This is a luminometer that measures the amount of light emitted from chemiluminescent reactions.
  • It is designed to be used with Gen-Probes’s, Amplified MTD, assays.
  • It efficiently stores up to 30 protocols and accommodates the throughput needs of most laboratories for diagnosis of Tuberculosis and other diseases.

Used for MTD Amplified Products - Direct detection of Mycobacteria using clinical specimens

BIORAD Gel DOC System

The Gel Doc XR+ System is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence and colorimetric detection.

Uses:

  • Gel and blot imaging and analysis are quick and accurate
  • Automated, hands-off routines;
  • Optimize the system at setup for image data that is always accurate, reproducible, and free of imaging artifacts
  • Comprehensive, automated quantitative analysis of protein and DNA samples

Our Team of Doctors

Dr Atul Thatai

National Head

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Dr Aditi Sarkar

Sr. Research Scientist

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Dr Sandeep Sharma

Research Scientist

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Dr Priyanka Agarwal

Research Scientist

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Dr Jasbeer Chhabra

Sr. Research Scientist

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The Department Staff Structure

  • 05

    Consultant & Scientists
  • 15

    Lab Technicians
  • 01

    Lab Executives

Available Tests

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Molecular Diagnostics